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PEER-REVIEWED PAPERS (Published or In Press):

1.   Stahelin, R.V. and Cho, W. “Membrane Binding Analysis of C1 and C2 Domains    Using Surface Plasmon Resonance” (2001) Biophys. J., 80:1, 534a.

2.   Bittova, L., Stahelin, R.V., and Cho, W. “Roles of Ionic Residues of the C1 Domain in Protein Kinase C-α Activation and the Origin of Phosphatidylserine Specificity” (2001) J. Biol. Chem., 276, 4218-4226.

3.    Stahelin, R.V. and Cho, W. “Differential Roles of Ionic, Aliphatic, and Aromatic Residues in Membrane-Protein Interactions: A Surface Plasmon Resonance Study on Phospholipases A2” (2001) Biochemistry, 40, 4672-4678.

4.    Stahelin, R.V. and Cho, W. “Roles of Ca2+ Ions in the Membrane Binding of C2 Domains” (2001) Biochem. J., 359, 679-685.

5.   Stahelin, R.V., Long, F., Diraviyam, K., Bruzik, K.S., Murray, D., and Cho, W. “Phosphatidylinositol-3-Phosphate Induces the Membrane Penetration of the FYVE Domains of Vps27p and Hrs” (2002) J. Biol. Chem., 277, 26379-26388.

6.   Karathanassis, D., Stahelin, R.V., Bravo, J., Perisic, O., Pacold, C.M., Cho, W., and Williams, R.L. “Binding of the PX Domain of p47phox to Phosphatidylinositol-3,4-bisphosphate and Phosphatidic Acid is Masked by and Intramolecular Interaction (2002) EMBO J., 21, 5057-5068.

7.    Stahelin, R.V., Forslund, R.E., Wink, D.J., and Cho, W. “Devlopment of a Biochemistry Laboratory Course with a Project-Oriented Goal” (2003) Biochemistry and Molecular Biology Education, 31, 106-112.

8.    Stahelin, R.V., Rafter, J.D., Das, S., and Cho, W. “A Molecular Basis for Differential Subcellular Localization of C2 Domains of Protein Kinase C-α and Cytosolic Phospholipase A2” (2003) J. Biol. Chem., 278, 12452-12460.

9.   Diraviyam, K., Stahelin, R.V., Cho, W., and Murray, D. “Computer Modeling of the Membrane Interactions of FYVE Domains” (2003) J. Mol. Biol., 328, 721-736.

10.    Stahelin, R.V., Burian, A., Murray, D., Bruzik, K.S., and Cho, W. “Membrane Binding Mechanisms of the NADPH Oxidase PX Domains” (2003) J. Biol. Chem., 278, 14469-14479.

11.    Stahelin, R.V., Long, F., Peter, B.J., Murray, D., De Camilli, P., McMahon, H.T., and Cho, W. “Contrasting Membrane Interaction Mechanisms of AP180 and N-terminal Homology (ANTH) and Epsin N-terminal Homology (ENTH) Domains” (2003) J. Biol. Chem., 278, 28993-28999.

12.   Ananthanarayanan, B., Stahelin, R.V., Digman, M.A., and Cho, W. “Activation Mechanisms of Conventional Protein Kinase C Isoforms are Determined by the Ligand Affinity and Conformational Flexibility of Their C1 Domains” (2003) J. Biol. Chem., 278, 46886-46894.

13.   Malkova, S., Stahelin, R.V., Long, F., Pingali, S.V., Cho, W., and Schlossman, M.L. “X-ray Reflectivity Studies of cPLA2-C2 Domains Adsorbed onto Langmuir Monolayers of SOPC” (2004) Biophys. J., 86:1, 377a.

14.   Stahelin, R.V., Digman, M.A., Medkova, M., Ananthanarayanan, B., Rafter, J.D., Melowic, H.R., and Cho, W. “Mechanism of Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cδ” (2004) J. Biol. Chem., 279, 29501-29512.

15.   Blatner, N.R., Stahelin, R.V., Diraviyam, K., Hawkins, P.T., Hong, W., Murray, D., and Cho, W. “The Molecular Basis of the Differential Subcellular Localization of FYVE Domains” (2004) J. Biol. Chem., 279, 53818-53827.

16.   Stahelin, R.V., Ananthanarayanan, B., Blatner, N.R., Singh, S., Bruzik, K.S., Murray, D., and Cho, W. “Mechanism of Membrane Binding of the Phospholipase D1 PX Domain” (2004) J. Biol. Chem., 279, 54918-54926.

17.   Subramanian, P., Stahelin, R.V., Szulc, Z., Bielawska, A., Cho, W., and Chalfant, C.E. “Ceramide-1-Phosphate Acts as a Positive Allosteric Activator of Cytosolic Phospholipase A2 and Enhances the Interaction of the Enzyme with Phosphatidylcholine” (2005) J. Biol. Chem., 280, 17601-17607.

18.    Stahelin, R.V., Digman, M.A., Medkova, M., Ananthanarayanan, B., Melowic, H.R., Rafter, J.D., and Cho, W. “Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cε: Mechanistic Differences Between PKCδ and ε” (2005) J. Biol. Chem., 280, 19784-19793.

19.   Malkova, S., Long, F., Stahelin, R.V., Pingali, S.V., Murray, D., Cho, W., and Schlossman, M.L. “X-ray Reflectivity Studies of cPLA2-C2 Domains Determine its Depth of Penetration and Membrane Orientation” (2005) Biophys. J., 89, 1861-1873.

20.    Stahelin, R.V., Wang, J., Blatner, N.R., Rafter, J.D., Murray, D., and Cho, W. “The Origin of C1 and C2 Domain Interactions of PKC-α In Vitro and In Vivo” (2005) J. Biol. Chem., 280, 36452-36463.

21.    Stahelin, R.V., Hwang, J.H., Kim, J.H. , Park, Z.Y., Johnson, K.R., Obeid, L., and Cho, W. “The Mechanism of the Subcellular Localization of Human Sphingosine Kinase 1” (2005) J. Biol. Chem., 280, 43030-43038.

22.   Bhardwaj, N., Stahelin, R.V., Langois, R.E., Cho, W., and Lu, H. “Structural Bioinformatics Prediction of Membrane-Binding Proteins” (2006) J. Mol. Biol., 359, 486-495.

23.   Mallikaratchy, P., Stahelin, R.V., Cao, C., Cho, W., and Tan, W. “Selection of High Affinity DNA Ligands for Protein Kinase C-δ” (2006) Chem. Commun., 2006, 3229-31.

24.   Malkova, S., Stahelin, R.V., Pingali, S.V., Cho, W., and Schlossman, M.L. “Structure of p40phox-PX Bound to a Monolayer of SOPC/SOPS/DPPtdIns(3)P” (2006) Biochemistry, 45, 13566-13575.

25.   Lee, S., Kovacs, J, Stahelin, R.V., Cheever, M.L., Burd, C., Cho, W., and Kutateladze, T.G. “Molecular Mechanisms of Membrane Docking by the Vam7p PX Domain” (2006) J. Biol. Chem., 281, 37091-37101.

26.    Stahelin, R.V., Karathanassis, D., Bruzik, K.S., Waterfield, M.D., Bravo, J., Williams, R.L., and Cho, W. “Structural and Membrane Binding Analysis of the PX Domain of Phosphoinositide 3-Kinase-C2α” (2006) J. Biol. Chem., 281, 39936-39406.

27.   Lamour, N.F., Stahelin, R.V., Wijesinghe, D.S., Maceyka, M., Wang, E., Allegood, J.C., Cho, W., Merrill, A.H., and Chalfant, C.E. “Ceramide Kinase Utilizes Ceramide Provided by Ceramide Transport Protein” (2007) J. Lipid Res., 48, 1293-1304.
        Highlighted in ASBMB Today, July 2007

28.    Stahelin, R.V., Subramanian, P., Vora, M., Cho, W., and Chalfant, C.E. “Ceramide-1-Phosphate Binds Group IVA Cytosolic Phospholipase A2 Via a Novel Site in the C2 Domain” (2007) J. Biol. Chem., 282, 20467-20474.

29.   Melowic, H.R., Stahelin, R.V., Blatner, N. R., Tian, W., Hayashi, K., Altman, A., and Cho, W. “Mechanism of Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cθ” (2007) J. Biol. 282, 21467-21476.

30.    Stahelin, R.V., Karathanassis, D., Murray, D., Perisic, O., Bruzik, K.S., Williams, R.L., and Cho, W. “Structural and Membrane Binding Analysis of the PX domain of Bem1p” (2007) J. Biol. Chem., in press.

31.   Bhardwaj, N., Stahelin, R.V., Cho, W., and Lui, H. “MeTaDoR: A Comprehensive Resource for Membrane Targeting Domains Hosted By Peripheral Proteins” (2007) Bioinformatics, in press.

REVIEWS BOOK/CHAPTERS

32.    Cho, W., Bittova, L., and Stahelin, R.V. “In vitro Membrane Binding Assays for Peripheral Proteins” (2001) Anal. Biochem., 296, 153-161.

33.    Cho, W., Digman, M.A., Ananthanarayanan, B., and Stahelin, R.V. “Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms” (2003) Methods in Molecular Biology, vol. 233: Protein Kinase C Protocols, Ed., Newton, A.C., K. Humana Press, Totowa, New Jersey 291-298.

34.    Cho, W., and Stahelin, R.V. “Membrane-Protein Interactions in Membrane Trafficking and Signal Transduction” (2005) Annu. Rev. Biophys. Biomol. Struct. 34:119-151.

35.    Cho, W. and Stahelin, R.V. “In Vitro and Cellular Membrane Binding Mechanisms of Membrane Targeting Domains” (2005) In “Protein-Lipid Interactions”, Tamm, L.K. Ed., Academic Press, N.Y., 369-401.

36.    Cho, W. and Stahelin, R.V. “Membrane Binding and Subcellular Localization of C2 Domains” (2006) Biochim. Biophys. Acta, 1761, 838-49.